"Marked for Destruction by Dr. Doom!" (21 pages)
Doctor Doom, fresh off his defeat by the Fantastic Four, is looking to form a new strategy for the demise of the FF. He invites Spider-Man to his New York City hideout to suggest a partnership, which Spidey rejects. Not to be denied, Doom revises his strategy to one where he holds Spider-Man hostage to bend the Fantastic Four to his will. The plan goes afoul when Doom inadvertently captures Flash Thompson, who was in the middle of a prank to scare Peter Parker by posing as Spider-Man. Now Peter is faced with having to rescue his school rival from the menace of Doctor Doom. Also in this issue, the relationship between Peter Parker and Betty Brant starts to heat up.
Cameo appearance by the Fantastic Four.
Reprinted in Amazing Spider-Man Annual #2, Marvel Tales (1966) #142, and Spider-Man Classics #6.
Collected in Spider-Man: Doctor Octopus.